Rit Landbúnaðardeildar : B-flokkur - 01.05.1947, Side 8

Rit Landbúnaðardeildar : B-flokkur - 01.05.1947, Side 8
6 South-Sweden. During the last summer research was carried out on rather large material of C. edentula from the south- east beaches of the bay Faxaflói in South-West of Iceland. That material was fixedin Carnoy 3:1 and stained in acetic-orcein (cf. Darlington and La Cour, 1942) and smeared in the same way as described by Löve (1943). The results of the investiga- tion are given below. 2. Cytological results. a. Cakile maritima L. The European species C. maritima was found to be diploid with 2n = 18, n = 9 chromosomes in our material from South-Sweden. The same number had previously heen determined by Wulff (1937) in material from Schleswig-Holstein and by Hagerup (1941) in inaterial from Denmark. Our observations were made on root-tips, but no examina- tions were made by the present writers of the ineiotic hehaviour of this species. b. Cakile edentula (Bigel.) Hook. The chromosome number of the Icelandic material of C. edentula was found to be 2n = 36, n = 18, i.e. the species is to be regarded as a tetraploid. The meiotic behaviour was examined rather thoroughly, although no observation was made on the earlier stages of prophase, as the material was not easily stained at these stages in acetic-orcein. Good slides were obtained at diakinesis and the following stages, but only few observations were made at the stages before metaphase-I. At diakinesis the bivalents are mainly united by one or two chiasmata, and some few multivalents were found in every cell studied. The terminalization was complete or almost complete. At metaphase-I most of the chromosomes are found as bivalents but in every cell studied there were one or more quadrivalents observable. Analysis of metaphase-I in side-view is not difficult in the material if studied iinmediately, but as the slides were not thoroughly analysed at once, only a relatively low number of cells were totally investigated. In Fig. 1 a typical metaphase-I with 4 quadrivalents is shown, and in Fig. 2 five quadrivalents from another cell are depicted. A metaphase-I plate with two quadrivalents in polar view is shown in Fig. 3. The chromo- some pairing was analysed in 294 cells with the results given in Table 1. As shown in that table univalents are observed in only 6 cells, i.e. in about

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