Íslenskar landbúnaðarrannsóknir - 01.03.1970, Qupperneq 38

Íslenskar landbúnaðarrannsóknir - 01.03.1970, Qupperneq 38
36 ÍSLENZKAR LANDBÚNAÐARRANNSÓKNIR The genotyping was based on the as- sumption that the colours could be ex- plained in terms of genes at the three loci, A, B and S, as describecl previously, p. 26. For genotyping individual ewes their own colour was the first criterion. White ewes were always assumed to carry A1( and if they came from white parents and had never given any nonwhite progeny they could not be genotyped any further. Their genotype was then written as 1........., where the dots represent unknown genes. During punching, only the first of the 6 columns in the card reserved for the geno- type was punched and the others left blank. II a wliite ewe was known to be the daughter of a black sire, her genotype was punched as 15 ...., if nothing was known about the sire’s genotype with respect to brown colour and white markings, and if no further information about the ewe’s genotype could be obtained from her own progeny. For a white ewe out of a white dam and a brown sire with white mark- ings the genotype was punched as 15.2.2. the blanks representing the unknown genes at loci B and S, which she had received from the dam. If the tliird lamb of a ewe of this parentage had a brown sire with white markings and was black without white markings, this lamb woulcl be taken as a proof that the ewe was of the geno- type A^AgB^B^S^So, which was then punched as 151212. This would then be punched as her genotype in all cards carry- ing records made one year after this dis- covery and later. For records made up to and including the discovery of the previ- ously unknown genes, the same genotype would also be punched in the cards, but an X (11-punch) would be punched above the newly discoverecl genes iu all cards up to and including the year of discovery. l’he first three cards for the ewe in the above example would then have had the X X genotypes punched as 151212 (or 15J2J2)> while in all subsequent cards the genotype would be punched as 151212. The above distinction between genes known prior to and subsequent to a parti- cular mating was made in order to use in- formation after the particular mating as a mating of a known genotype. Matings prior to the discovery were on the other hand sometimes used in connection with genotyping of rams. When tlie ewes had been genotyped and all necessary non-numerical information cod- ed the data were punched and the punched cards checked as described previously. During the punching, no information about the genotype of the ram was punch- ed into the cards. After the punching was completed, liowever, the cards were sorted on main colour of lamb, genotype of dam, year of record and ram number, and only one locus of genotype of dam was taken into account at a time. After the first sorting, the cards were available in order according to ram, year on record sorted within each ram, geno- type of ewe at the A-locus sorted within year with zone punching in genotype sup- pressed, and colour of lambs sorted within genotype of ewe. The cards were now run through a tabulator and counts made for lambs of each colour within genotype of ewe, record year and ram number. From these tables and from the colour of the sire and the information about the colour of his par- ents, his genotype at locus A was deter- mined. When all rariis had thus been geno- typed with respect to the A-locus, the cards were again sorted in a similar way, but now with respect to the B-locus,! and fin- ally for the S-locus. When rams were geno- typed with respect to the S-locus, the cards were sorted on 3rd and 4th digit of lamb colour, and not on main colour.
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