Læknablaðið : fylgirit - 01.05.1978, Blaðsíða 149

bacteria. In the experiment with Proteus
mirabilis it was possible to remove much of 3.
the B12 by adding either EDTA in a final con-
centration of 0.25 M or LBP to the bacteria.
This effect was demonstrable even 1 h. after 4.
the addition of B12 in this particular experiment.
In experiments with E. coli and klebsiella it 5.
was possible to remove B12 by adding LBP or
EDTA within 1 min., but this could not be done 6.
after 1 h. Whenever EDTA was able to remove 7.
pre-added B12 from the bacteria, BI2 could also
be removed by LBP.
The present results, whieh are preliminary,
confirm the idea that LBP might efficiently 8.
compete with Bi^-uptaking bacteria for B12 in
the medium. Thus, LBP could inhibit a 9.
presumptive synergism between Bi^-producing
and Bi2-uptaking microorganisms in the intes- 10.
tinal Qora. Experiments are in progress to
reinforce this proposed mechanism by oral 11.
administration of LBP to patients with disturbed
intestinal fiora. 12.
Aoknowledgem ents: 13.
This work was supported by grants from
Konung Gustaf V:s 80-ársfond, the Swedish
Association against Rheumatic Diseases, the 15
Swedish Medical Research Council (19X-3109),
and funds of the Karolinska Institutet.
16.
References:
1. Allen, R.H. & Mehlman, C.S. , J. Biol. 17.
Chem. 248:3670, 1973.
2. Coates, M.E. , Vitamin Bl2, Academic
Press, New York and London, 1968, p. 243.
Dellipiani, A.W. , Samson, R.R. & Gird-
wood, R.H., Am. J. Dig. Dis. 13:
718, 1968.
Ford, J.E., Br. J. iNutr. 31 : 243,
1974.
Ford, J.E. & Hutner, S.H. , Vitamins
and Hormones, 13:101, 1955.
Gilbert, H.S. Blood 44: 926, 1974.
Glass, G.B.J. , Gastric instrinsic
factor and other vitamin B12
binders, Georg Thieme Publ., Stuttgart,
1974.
Goldman, A.S. & Smith, C.W. ,
J. Pediatr. 82:1082, 1973.
Gullberg, R., 639, 1972. Scand. J. Haemat. , 9:
Gullberg, R., 8:497, 1973. Scan d. J. Gastroent,
Gullberg, R., 9:287, 1974. Sc and. J. Gastroent,
Kane, S.P. & Peters, T.J. , Clin. S ci.
Mol. M ed . 49 :171 , 1975 .
Nakonecza, I. , Forbes, J.C . & Rogers,
K.S., A m. J . Path . 57: 523, 1969.
Olhagen, B. Ann. Clin. 1975. Res. 7:119,
Schjönsby, H. , Drasar, B.S. ., Tabaqchali,
S. & Booth, C 8:41, 1973. .C., Scand. J. Gastroent
Taylor, R.T., Norrel, S.A. & Hanna, M. L.
Arch. Biochem. Biophys., 148:
366, 1972.
White, J. C., Di Girolami, P.M., Fu. L.M. ,
Preston, Y.A. & Bradbeer, C. , J. Biol.
Chem . 24 8; 3978, 1973.
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