Læknablaðið : fylgirit - 01.05.1978, Page 149

bacteria. In the experiment with Proteus
mirabilis it was possible to remove much of 3.
the B12 by adding either EDTA in a final con-
centration of 0.25 M or LBP to the bacteria.
This effect was demonstrable even 1 h. after 4.
the addition of B12 in this particular experiment.
In experiments with E. coli and klebsiella it 5.
was possible to remove B12 by adding LBP or
EDTA within 1 min., but this could not be done 6.
after 1 h. Whenever EDTA was able to remove 7.
pre-added B12 from the bacteria, BI2 could also
be removed by LBP.
The present results, whieh are preliminary,
confirm the idea that LBP might efficiently 8.
compete with Bi^-uptaking bacteria for B12 in
the medium. Thus, LBP could inhibit a 9.
presumptive synergism between Bi^-producing
and Bi2-uptaking microorganisms in the intes- 10.
tinal Qora. Experiments are in progress to
reinforce this proposed mechanism by oral 11.
administration of LBP to patients with disturbed
intestinal fiora. 12.
Aoknowledgem ents: 13.
This work was supported by grants from
Konung Gustaf V:s 80-ársfond, the Swedish
Association against Rheumatic Diseases, the 15
Swedish Medical Research Council (19X-3109),
and funds of the Karolinska Institutet.
16.
References:
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Chem. 248:3670, 1973.
2. Coates, M.E. , Vitamin Bl2, Academic
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Dellipiani, A.W. , Samson, R.R. & Gird-
wood, R.H., Am. J. Dig. Dis. 13:
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Ford, J.E., Br. J. iNutr. 31 : 243,
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Ford, J.E. & Hutner, S.H. , Vitamins
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Gilbert, H.S. Blood 44: 926, 1974.
Glass, G.B.J. , Gastric instrinsic
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Goldman, A.S. & Smith, C.W. ,
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