Læknablaðið : fylgirit - 01.05.1978, Page 152

TABLE I The peroxidase-activity in mononuclear leukocytes
separated by Isopaque-Ficoll gradient centrifugation.
Technique Peroxidase- ■positive cells
Mean Range
Mod. Graham Knoll 20.8 (16.5-23.5)
Kaplow 20.7 (12.9-27.9)
Leder 14.7 (12.1-18.5)
monocyte morphology were moBt often very few
and always less than 1% irrespective of the
methods used.
Per oxidas e-act iv ity in m it ogen-stimu -
lated lymphocyte culture
After incubation of Isopaque-Ficoll separated
mononuclear leukocytes in vitro for 3 days, the
cell suspension was depleted of peroxidase
activity, whether they were cultured with mitogens
or not.
The effect of nylon fibre column
fractionation on the peroxidase posi-
tive cells
In the nylon fibre fractionation experiments
four different blood donors were tested. Before
fractionation of the Isopaque/Ficoll separated
cells, the peroxidase positive cells were on
average 19% (range 197o-29/o). (Fig. 1). In
contrast, cell suspensions fractionated on a nylon
column contained practically no peroxidase posi-
tive cells (< 0. 9$) nor cells with monocytic
morphology as determined by differential count-
ing of the cell suspensions stained with May-
Gríinwalds Giemsa. After fractionations the
percentage of EA-RFC was slightly reduced indi-
cating that a small proportion of the peroxidase
positive cells might also form EA-rosettes. This
was supported by peroxidase-staining of the EA-
rosette preparations made before nylon fibre
fractionation which showed that on an average.
23.6% of the EA-rosette forming cells were
peroxidase positive (range 21%-28/o). After
nylon fibre fractionation no EA-rosettes were
peroxidase positive (Fig. 2a) and the cytotoxic
activity of the cell suspensions was actually
higher, in all four experiments (mean CI 69.0,
and range 54.1-80.4) than before fractionation.
(Mean CI 39.4 and range 22.5-69.3) (Fig. 2b).
The effect of EA-rosette s edim ent a t i on
on the peroxidase positive cells
In several experiments where EA-rosette sedi-
mentation was performed, quite different results
from those with nylon fibre fractionation, was
Ly Ly-C
Fig. 1 Peroxidase activity in cells separted by
Isopaque-Ficoll gradient centrifugation
(Ly) and then fractionated on nylon fibre
column (Ly-C). Broken horizontal lines
show the mean values.
pox pos = peroxidase positive cells
pox neg = peraxidase negative cells
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