Fróðskaparrit - 01.07.2004, Page 37

VAL AV LÍVØKI HJÁ ARBUSKULSOPPUM
í FØROYUM SAMMETT VIÐ AÐRASTAÐNI
35
Canonical Discriminant Functions
SITE
□ Group Centroids
a Sourhope
• Velbastað
0 Somfelli
Function 1
study and also in most of the host plants.
The phylogenetic tree (Fig. 1) is based
on the region amplified by the AM2 primer;
meaning that the basepairs between AM2
and AM1 are omitted. However, since most
of the variation lies between 80-300 bp
from the 5’ (NS31) of the PCR product,
most of the variation should be retained
(Helgason et al., 1999).
Fig. 2. Canonical Discriminant analysis of the T-
RLFP valuesfrom 2 Faroese sites and one Scotish, all
grazed grassland (Sornfelli: north-facing, high alti-
tude; Velbastað: south-facing, low altitude; Sourhope:
scottish, low altitude). The three sites have clearly dis-
tinct AM communities.
The T-RFLP results (Fig. 2) indicate that
the fungal communities in the sites are dis-
tinct. In contrast to the clone-work only
Hiníl was used, and only the strand from
NS31 considered. This is due to the fact
that a very large fraction of the AM fungi
have a Hinfl site directly after the AMl
primer, and therefore no results are ob-
tained from the AM1 -labelled end. Previ-
ous attempts also showed that Hsp92ll
didn’t give any additional information,
since most of the types were cut into the
same pattem.
Discussion
Results from clones
Only one previous molecular study of AM
fungi appears to have used Agrostis capil-
laris as the target plant: Vandenkoornhuyse
(2002) examined A. capillaris and Trifoli-
Fig. 1. Phylogenetic (Neighbour-joining) tree showing all the glomalian fungifound in Agrostis capillaris. The
numbers in circles indicate where else these types have beenfound.
I: Sequences obtained from Faroes
2: Sequences obtainedfrom Sourhope (Genbank: accession nos: AF437637-437723, Vandenkooornhuyse et al„
2002)
2: Spores from Shetland identified as the named type (Watling, 1992)
4: Sequences obtainedfrom a Tropical Forest (Genbank: accession nos: AY129570-129633; Husband et al„ 2002)
5: Sequences obtained from a Britisli Forest (Genbank accession nos: AF131020-131055; Helgason et al., 1999)
6: Sequences obtainedfrom a British Forest (Genbank accession nos: AF485861-485890; Helgason et al., 2002)
7: Sequences obtainedfrom identified spores (Genbank accession nos on the graph; Schiifiler et ai, 2001)
8: Sequence obtainedfrom library culture (see Helgason et ai, 1999)
Abbreviations: Glo: Glomus; Acau: Acaulospora; Scu: Scutellospora; Paraglo: Paraglomus; Archaeo: Ar-
chaeospora.