Náttúrufræðingurinn - 1970, Blaðsíða 43

NÁTTÚRUFRÆÐIN GU R1 N N
185
Vessel-buffer
Borate-buffer pH 8,65 (Poulick, modificd).
I-Iyclrolysed starcli (Connaught) was used, 11—14 g/100 ml.
Nigrosine-Amido-Black in methanol-acetic acid-water was used for staining
general proteins. Coupling of naplithyl compouuds with Fast Garnet G.B.C.
was used to stain esterases.
Ptarmigans were either shot or trapped, bled when possible and kept frozen
until the samples were dealt with. Tissues were homogenised with ultra-sonic
waves in aliquots of distilled water.
Results
Serurn
Samples were obtained from 130 L. m. islandorum.
The general protein-pattern (Fig. 2) reveals eight distinct bands, and by
concentrating the serum more bands could be brought up. Fractious 1, 2, 3
are prealbumins, 7 and 8 are transferrins.
The esterase-zymogram (Fig. 3) comprises six zones which can in turn be
split up into individual fractions. Zone C has fractions 3, 4 and 5 and all frac-
tions were present in all samples (130). Zones E and F are diffused zones and
are affected by neuraminidase, other zones are not affected.
Liveresterases (Fig. 4)
Samples were obtained from 2 L. m. captus (Greenland), 48 L. m. island-
orum (Iceland) and 6 L. m. mutus (Norway). One Willow Ptarmigan (L.
lagopus) from Norway was used for comparison.
The electrophoretic pattern:
Zone A, fraction 1 is common to all L. rnutus and la is the corresponding
fraction in L. lagopus.
Zone B, fractions 2 and 3 are found in L. mutus captus and L. mutus is-
landorum, but is absent in L. mutus mutus.
Zone C, fraction 4—9. Here we find differences again between the European
and American groups. The Norwegian samples had strong fractions 4, 5 and 6.
The ptarmigan from Iceland and Greenland had weak fraction 4, fractions 5,
6 and 7 were strong, fraction 8 was strong in only a few samples, and 9 weak
when it was present. Zone C is called CF in the Norwegian samples, because
of its distinctly different appearance. The following specificity tests were
carried out on the liveresterases:
a) Reaction to different substrates. All zones reacted to 1-naphtliyl-acetate
and 2-naphthyl-acetate. Zones B and C reacted to Naphtol-AS-acetate, but z.one
A did not (Fig. 4, T). No zone reacted to 6-bromo-2-carbonaphthoxy choline
iodide.
b) Effects of neuraminidase (Fig. 5). Zones A and B are not affected, but
tlie fractions in zone C are slowed down.