Fróðskaparrit - 31.12.2000, Side 174

178
PARASITES IN SHEEPIN THE FAROE ISLANDS
Strongyles, Nematodirus Trichuris and Eimeria Dictyocaulus and Muellerius Fasciola hepatica
Winter Spring Autumn Winter Spring Autumn Winter Spring Autumn
Lamb 24 35 79 24 34 64 24 31 0
Ewe 35 28 25 31 28 15 34 27 0
Table 1. Number offaecal samples collected in 1999. The table groups the samples in season, parasites searched
for, and samples from lambs and ewes.
Talva 1. Tal av tøðvertum tiknir í 1999. Talvan býtir vertirnar sunđur í árstíð, hvørjir sníkar leitað hevur verið
eftir, og um vertimir eru av lombum ella óm.
last treated with anthelmintics and fluki-
cides in the autumn of 1998. The treatment
was levamisole against nematodes and tri-
clabendazole against Fasciola hepatica.
The sheep examined in the autumn of 1999,
were last treated with anthelmintics -
febantel - in the summer of 1999. The
sheep examined in the winter of 1999, were
last treated with anthelmintics and fluki-
cides - levamisole and triclabendazole - in
the autumn of 1998.
Procedures
Abomasum. content
The abomasum was placed in a tray, and
split open along the curvatura major. It was
emptied, and the mucous membrane was
thoroughly washed. The mucous mem-
brane was rubbed gently, to be sure to col-
lect as many as possible of the adult nema-
todes. Ten percent of the total abomasal
content was then poured through a sieve
(mesh size of 106 pm). The material was
diluted to a known volume of water and
coloured and fixed with iodine solution.
An aliquot of 1 to 10% - percentage de-
pending on concentration of nematodes -
of the retained material was examined un-
der a stereomicroscope and any nematode
present was collected and grouped accord-
ing to gross anatomy. The total number of
nematodes in the abomasum was calculated
from total sample and aliquots obtained.
Abomasum. mucosal membrane
The mucosal membrane of the abomasum
was scraped off and weighed. It was mixed
with a digestion solution composed of 1
litre of water, 8 grams of pepsin, and 150
nrl of hydrochloric acid 1N, and left to in-
cubate in 40°C for 6-8 hours. The solution
was repeatedly stirred during the digestion
period. The digested material was then
poured through a sieve (mesh size of 38
pm), which retained any larvae present.
The number of larvae was determined.
Ileum and jejunum
The small intestine was split in its total
length, and the content was emptied into a
tray. The mucous membrane was washed
in the same way as for the abomasum to re-
cover all nematodes present. An aliquot of
10% was poured through a sieve (mesh size
of 106 pm). As for the content of the abo-
masum, an aliquot of 1 to 10% of the re-