Fróðskaparrit - 01.01.2001, Page 34

Fróðskaparrit - 01.01.2001, Page 34
38 CORALLINE RED ALGAE (CORALLINALES, RHODOPHYTA) OF THE FAROE ISLANDS Material and methods Algae were collected selectively by hand from the littoral to 30 m depth, in the sub- littoral by SCUBA diving; in addition ma- terial was obtained by dredging at a few of the 265 stations. Collections were made at all seasons, but less in the winter months, with only a small collection from January, and none from November, December and March. The localities visited were distrib- uted on most of the larger islands and in- cluded places on the open coasts with dif- ferent exposure to waves as well as more or less sheltered places in the fjords. Informa- tion about the localities with details of col- lection dates, depth and geographical coor- dinates for each station, are available in Sørensen et al. (2001) while amap showing the locations is available in Nielsen and Gunnarsson (2001). The main part of the obtained material was dried immediately after collection, but a smaller fraction was stored in a 7:2:1 solution of ethanol. dis- tilled water and gl'ycerol to keep it moist. For identification it is often necessary to decalcify and section the specimens. The material was decalcified overnight in dilute (2-5%) acetic acid, then rinsed once in distilled water. Part of the material was embedded in Cryo Embed (AX-LAB A/S, Copenhagen, Denmark). Sections of 8 pm were cut on a Reichert-Jung 2800 Frigocut microtome (freeze microtome), collected on a slide, stained with few drops of 1% Cotton Blue dissolved in lacto glyc- erol (875 ml lactic acid, 63 ml glycerol, 62 ml distilled water) and mounted in 35-50 % KARO® (com syrup). Another part was embedded in LR-White resin (London Resin Company, UK), cut on a Reichert- Jung 2050 Supercut microtome in sections of 7 pm and mounted for permanent slides in Eukitt (Bie and Bemtsen, Denmark). This method is modified after Woelkerling (1988); for methodological details see Díiwel and Wegeberg (1996). Voucher specimens are kept in the Botanical Museum, Copenhagen (C) with duplicate collections in the Museum of Natural History, Tórshavn and the Marine Research Institute, Reykjavik, Iceland. Results Taxonomy and nomenclature generally fol- low Chamberlain and Irvine (1994a, b) and Irvine and Johansen (1994). Authors of plant names are abbreviated according to Brummit and Powell (1992). Included in the list as synonyms are names used by Børgesen (1902), Irvine (1982) and Sim- mons (1897). For each species at least one reference indicates a good description, il- lustration, or other specific information. The BIOFAR stations where the respective species were collected are listed as well as notes recording depth distribution, month(s) of collection and observations of reproductive structures. The list of species includes the following symbols: *: new or previously unpublished records for the Faroe Islands. (): collection from a wide depth range. Depth distribution: Only handpicked mate- rial included.
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