Læknablaðið - 15.09.1993, Page 46

296
LÆKNABLAÐIÐ
label OXANOH a constant concentration was
found in the venous blood from the kidney.
Determination of OXANO* concentration in
the venous blood was therefore performed
at least 2 min after the start of the intra-
arterial OXANOH infusion. At reperfusion
blood flow returned to pre-ischaemic values
after a few minutes in all groups. The relative
production of radicals at reperfusion after one
hour of ischaemia without pretreatment was
increased by approximately 100 per cent for
at least one hour. In all treated groups except
the mannitol group, a significant reduction in
radical production could be seen compared to
untreated controls. The experiments on renal
function showed that 60 min of ischaemia
caused a drastic decrease in kidney function.
Desferrioxamine pretreatment appeared to
be more effective than inannitol alone in
preserving glomerular function
after ischaemia. Oxypurinol, or IC-SOD
and catalase in combination with mannitol
were not more effective than mannitol alone.
Concerning tubular function, combined
pretretment with mannitol, EC-SOD and
catalase, or mannitol and oxypurinol offered
certain advantages over mannitol pretreatment
alone.
Conclnsion: The spin trap technique using
OXANOH as a spin trap can be used
effectively to determine production of radicals
in rabbit kidneys. Pretreatment against radical
production by oxypurinol, IC-SOD, EC-
SOD, heparin or desferrioxamine reduces
radical production significantly. Several
of the different substances that intervene
against radical production have been shown
to improve kidney function after ischaemia
and reperfusion.