Læknablaðið - 15.09.1993, Page 44

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LÆKNABLAÐIÐ
untranslated region, in the coding sequence,
and in the 3’ flank did not significantly affect
PNP expression. Two fortuitous splice sites in
the PNP complementary strand co-localized
with the enhancer in intron-1. Shortening of
the 2.9 kb intron-1 to 855 bp had no effect on
PNP expression. Further shortening of intron-1
to 338 bp by eliminating both the enhancer
and the fortuitous splice sites abolished PNP
expression. Expression was partially restored
if the enhancer was reinserted in the reverse
orientation upstream of the PNP promoter or
in the 338 bp intron-1. Elimination of several
sequences associated with deletions during
retroviral-mediated gene transfer thus provided
PNP genes which retained function and may
be transduced intact once tested in a retroviral
vector.
ÞAKKIR
Höfundur naut syrkja meðal annars úr
Minningarsjóði Bergþóru Magnúsdóttur og
Jakobs J. Bjarnasonar og úr Vísindasjóði
Atlantshafsbandalagsins, til að vinna að
doktorsverkefninu.