Læknablaðið - 01.12.2015, Page 17

LÆKNAblaðið 2015/101 573
R A N N S Ó K N
ENgLISH SUMMArY
introduction: Acellular fish skin of the Atlantic cod (Gadus morhua) is
being used to treat chronic wounds. The prevalence of diabetes and
the comorbidity of chronic wounds is increasing globally. The aim of the
study was to assess the biocompatibility and biological characteristics
of acellular fish skin, important for tissue repair.
Materials and methods: The structure of the acellular fish skin was
examined with microscopy. Biocompatibility of the graft was conducted
by a specialized certified laboratory. Protein extracts from the material
were analyzed using gel electrophoresis. Cytokine levels were measured
with an enzyme linked immunosorbent assay (ELISA). Angiogenic
properties were assessed with a chick chorioallantoic membrane (chick
CAM) assay.
Results: The structure of acellular fish skin is porous and the material is
biocompatible. Electrophoresis revealed proteins around the size 115-
130 kDa, indicative of collagens. The material did not have significant
effect on IL-10, IL-12p40, IL-6 or TNF-α secretion from monocytes or
macrophages. Acellular fish skin has significant effect on angiogenesis
in the chick CAM assay.
Conclusion: The acellular fish skin is not toxic and is not likely to pro-
mote inflammatory responses. The graft contains collagen I, promotes
angiogenesis and supports cellular ingrowth. Compared to similar
products made from mammalian sources, acellular fish skin does not
confer a disease risk and contains more bioactive compounds, due to
less severe processing.
Decellularized fish skin: characteristics that support tissue repair
Skuli Magnusson1, Baldur Tumi Baldursson1,2, Hilmar Kjartansson1,2, Gudny Ella Thorlacius3, Ivar Axelsson1, ottar Rolfsson4, Petur Henry Petersen3,
Gudmundur Fertram Sigurjonsson1
1Kerecis Limited, Eyraragata 2, 400 Isafjordur, Iceland, 2Landspitali - The National University Hospital of Iceland, 3Faculty of Medicine, University of Iceland, 4Center for Systems Biology,
University of Iceland.
key words: Fish skin, extracellular matrix, acellular dermal graft, wound healing, tissue repair.
Correspondence: Baldur Tumi Baldursson, btb@kerecis.com
Heimildir
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