Læknablaðið - 15.05.1997, Page 9

LÆKNABLAÐIÐ 1997; 83
289
Lýsisneysla eykur lifun músa eftir
sýkingu með Klebsiella pneumoniae
Sigurður Björnsson1), Ingibjörg Harðardóttir2’, Eggert Gunnarsson3), Ásgeir Haraldsson1)
Björnsson S, Harðardóttir I, Gunnarsson E, Har-
aldsson A
Dietary fish-oil supplementation increases survival in
mice following Klebsiella pneumoniae infcction
Læknablaðið 1997; 83: 289-93
Introduction: Epidemiological studies have shown
that high intake of omega-3 fatty acids correlates
with low incidence of various diseases such as car-
diovascular diseases, asthma, diabetes mellitus and
various auto-immune disorders. It may therefore be
suggested that omega-3 fatty acids have substantial
impact on the immune system. Studies of the effect
of omega-3 fatty acids on survival in bacterial in-
fections have however been contradicting. A Dutch
study from 1991 showed increased survival in mice
fed fish-oil following infection with Klebsiella pneu-
moniae. Because of the contradicting results the
authors conducted a study with the hypothesis that
fish-oil intake increases survival after severe Kleb-
siella pneumoniae infection.
Methods: Thirty mice were fed fish-oil enriched diet
(10%), olive-oil enriched diet (10%) or standard
chow diet. After six weeks the mice were injected
intramuscularly with l.óxlO2 cfu of Klebsiella pneu-
moniae. The survival was measured at regular time
intervals for 120 hours.
Results: After 56 hours, 93% of the mice fed fish-oil
were alive and 68% and 40% of the mice fed olive-
Frá '’Barnaspítala Hringsins Landspítalanum, 2|Raunvís-
indastofnun Háskóla Islands, 3)Tilraunastöð Háskóla l’s-
lands í meinafræði að Keldum. Fyrirspurnir, bréfaskipti:
Ásgeir Haraldsson, Barnaspítala Hringsins, Landspítalan-
um, 101 Reykjavík; sími: 5601050; bréfsími: 560 1055; net-
fang: asgeir@rsp.is
Lykilorð: lýsi, lifun, sýkingar, mýs.
oil and standard chow respectively. The overall sur-
vival after 120 hours was 40% in the fish-oil group,
25% in the olive-oil group and 20% in the standard
group. The survival after 120 hours of the mice fed
the fish-oil enriched diet was significantly better
when compared to the two other groups
(p=0.0034).
Discussion: We conclude that fish-oil enriched diet
increases survival of NMRI mice following infection
with Klebsiella pneumoniae when compared to ol-
ive-oil supplementation or standard chaw. We
therefore conclude that the difference in survival is
probably based on the effect of omega-3 fatty acid
on the immune system. The immunological pathway
is still unknown and our results encourage further
studies.
Key words: fish-oil, survival, infection, mice, omega-3 fatty
acid.
Ágrip
Inngangur: Faraldsfræðilegar rannsóknir
hafa bent til þess að neysla lýsis, og þá fyrst og
fremst ómega-3 fitusýra, verndi gegn ýmsum
sjúkdómum til dæmis hjarta- og æðasjúkdóm-
um, astma, sykursýki og ýmsum sjálfnæmis-
sjúkdómum. Því er líklegt að ómega-3 fitusýrur
hafi víðtæk áhrif á stýringu ónæmiskerfisins.
Rannsóknir á áhrifum ómega-3 fitusýra á lifun
í alvarlegum bakteríusýkingum hafa gefið mis-
munandi niðurstöður. Hollensk rannsókn frá
1991 sýndi aukna lifun músa á lýsisbættu fæði í
alvarlegum bakteríusýkingum. Vegna þess að
niðurstöður hafa verið mismunandi var frekari
rannsókna þörf og í þessari rannsókn var sett
fram sama tilgáta og í hollensku rannsókninni,
að lýsisneysla væri verndandi í alvarlegum
Klebsiella pneumoniae bakteríusýkingum.
Aðferðir: Sett var upp dýratilraun þar sem