Læknablaðið - 15.04.1991, Page 12

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LÆKNABLAÐIÐ
SUMMARY
Detection of different types of human
papillomavirus in dysplastic lesions of the genital
tract.
All biopsies of the cervix, vagina, vulva and
penis submitted for histologic diagnosis to the
Department of Pathology at the University of
Iceland during a two month period were analyzed
for different types of human papillomavirus (HPV).
A total of 146 biopsies were analyzed. The study
was performed by in-situ hybridization using
DNA probes for HPV 6/11, 16/18 and 31/35/51.
HPV was detected in 30% of lesions exhibiting
morphologic evidence of HPV infection. In general
biopsies which were positive for HPV 6/11 were
without dysplasia or had low grade dysplasia
(CIN I) while biopsies positive for HPV 16/18
or HPV 31/35/51 usually were of higher grades
of dysplasia (CIN II or III). These results indicate
that HPV types 6/11, 16/18 and 31/35/51 ail cause
infections of the genital tract in Iceland and that
the association of dysplasia with different types
of HPV appears similar in Iceland as described
elsewhere. It is hoped that the methodology
described in this study may be useful in the clinical
setting.
ÞAKKIR
Rannsókn þessi var gerð á sýnum sem
fjölmargir læknar hafa sent Rannsóknastofu
Háskólans í meinafræði. Eru þeim færðar
bestu þakkir fyrir að hafa lagt til efniviðinn.
HEIMILDIR
1. Syrjanen KJ. Epidemiology of human papillomavirus
(HPV) infections and their associations with genital
squamous cell cancer. APMIS 1989; 97: 957-70.
2. Gissman L, de Villiers E-M, zur Hausen H. Analysis
of human genital warts (condylomata acuminata) and
other genital tumors for human papillomavirus type 6
DNA. Int J Cancer 1982; 29: 143-6.
3. Durst M, Gissman L, Ikenberg H, zur Hausen H.
A papillomavirus DNA from a cervical carcinoma
and its prevalence in cancer biopsy samples from
different geographic regions. Proc Natl Acad Sci
USA 1983; 80: 3812-4.
4. Gissman L, Wolnik L, Ikenberg H, Koldovsky U,
Schnurch HG, zur Hausen H. Human papillomavirus
types 6 and 11 DNA sequences in genital and
laryngeal papillomas and in some cervical cancers.
Proc Natl Acad Sci USA 1983; 80: 560-3.
5. Crum CP, Mitao M, Levine RU, Silverstein
S. Cervical papillomaviruses segregate within
morphologically distinct precancerous lesions. J Virol
1985; 54: 675-81.
6. Syrjanen K, Mantyjarvi R, Vayrynen M, et al. Human
papillomavirus (HPV) infections involved in the
neoplastic process of the uterine cervix as established
by prospective follow-up of 513 women for two
years. Eur J Gynaec Oncol 1987; 8: 5-16.
7. Syrjanen SM. Basic concepts and practical
applications of recombinant DNA techniques in
detection of human papillomavirus (HPV) infections.
APMIS 1990; 98: 95-110.
8. Richart RM. Cervical intraepithelial neoplasia. In:
Sommers SC, ed. Pathology Annual. New York:
Appleton-Century-Crofts, 1973: 301-28.
9. Crum CP, Ikenberg H, Richart RM, Gissman L.
Human papillomavirus type 16 and early cervical
neoplasia. N Engl J Med 1984; 310: 880-3.
10. Barrasso R, De Brux J, Croissant O, Orth G. High
prevalence of papillomavirus-associated penile
intraepithelial neoplasia in sexual partners of women
with cervical intraepithelial neoplasia. N Engl J Med
1987; 317: 916-23.
11. Wickenden C, Malcolm ADB, Byme M, Smith C,
Anderson MC, Coleman DV. Prevalence of HPV
DNA and viral copy numbers in cervical scrapes
from women with normal and abnormal cervices. J
Pathol 1987; 153: 127-35.
12. Reeves WC, Brinton LA, Carcia M, et al. Human
papillomavims infection and cervical cancer in Latin
America. N Engl J Med 1989; 320: 1437-41.
13. Nuovo GJ, Richart RM. A comparison of biotin- and
35S-based in-situ hybridization methodologies for
detection of human papillomavirus DNA. Lab Invest
1989; 61: 471-6.
14. Kiyabu MT, Shibata D, Amheim N, John Martin W,
Fitzgibbons PL. Detection of human papillomavims
in formalin-fixed, invasive squamous carcinomas
using the polymerase chain reaction. Am J Surg
Pathol 1989; 13: 221-4.
15. Ferenczy A, Winkler B. Cervical intraepithelial
neoplasia and condyloma. In: Kurman RJ, ed.
Blaustein’s Pathology of the Female Genital Tract.
3rd ed. New York: Springer-Verlag, 1987: 177-217.