Læknablaðið - 15.05.1998, Page 15

LÆKNABLAÐIÐ 1998; 84
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Fig. 2. Colonies produced by síimulation of progenitor cells isolated from umbilical cord blood with different cocktails of growth
factors.
A) BFU-E colony containing erythroblasts (magnification 275x).
B) CFU-GM colony containing granulocytes (smaller) and macrophages (bigger) (magnification 660x).
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GFU-GM
before
freezing
GFU-GM
after
freezing
BFU-E
before
freezing
BFU-E
after
freezing
Fig. 3. Colony formalion (CFU-GM and BFU-E) of mononuclear cells isolated from 19 umbilical cord blood samples before and
afterfreezing in liquid nitrogen. Bars represent tlte mean±standard error of the mean. No significant difference was found between
colony growtli before and after freezing (p=0.13 for CFU-GM; p=0.16 for BFU-E)
Table III. Results of CD34+ selection from five umbilical cord blood samples isolated with anti-CD34 coated DynaBeads.
(n) "MNC x106 Z1CD34 (%) CD34+ x105 Purity (% CD34) CD34+ x105 Yield
1 70 0.92 6.44 39.0 1.24 19.2
2 100 0.50 5.00 39.4 1.17 23.4
3 100 0.70 7.00 37.5 2.44 34.9
3)4 120 0.85 10.20 79.2 8.23 80.7
3I5 200 0.62 12.40 95.6 9.57 77.2
'] Number of mononuclear cells in the beginning.
■> Proportion of CD34+ cells in each MNC sample according to FACS analysis.
31 These samples were depleted of CD19+ cells before CD34 selection.