Læknaneminn - 01.10.1994, Blaðsíða 9

má aðra hvata en pólýmerasa til keðjufjölföldunar
DNA svo sem ligasa (ligasechain reaction). Bráðlega
munu koma á markað raðeiningar frá Abbott labora-
tories sem byggja á LCR (ligase chain reaction).
Fyrirtækið Organon teknika hefur þróað mark-
mögnunarkerfi sem notar þrjá hvata til fjölföldunar á
DNA eða RNA við lágt og jafnt hitastig sem nefnist
NASBA. Aukmarkmögnunarkerfanna(tai'getampli-
fication systems) hafa verið þróuð annars konar kerfi
sem þjóna hliðstæðum tilgangi. Má þar nefna s vonefnd
merkismögnunarkerfi (signal amplification systems)
ogkannamögnunakerfi (probeamplificationsystems).
Hvað sem öðru líður er líklegt að vænta megi
byltingarkenndra breytinga á því verklagi og starfs-
aðferðum sem tíðkast hafa lengi á sýklafræðideildum.
HEIMILDIR
1. Avery OT, MacLeod CM, McCarty M. Studies on the
chemical nature of the substance including
transformation of pneumococcal types: induction of
transformation by a desoxyribonucleic acid fraction
isolated from pneumococcus type III. J Exp Med
1944; 79: 137-158.
2. Eisenstein BI. The polymerase chain reaction: A new
method of using molecular genetics for medical
diagnosis. N Engl J Med 1990; 322: 415-420.
3. Mullis KB. The unusual origin of the polymerase
chain reaction. Sci Am 1990 (Apr.); 262: 56-65.
4. Saiki RK, Scharf S, Faloona F, Mullis KB, Horn GT,
Erlich HA, Arnheim M. Enzymatic amplification of B-
globin genomic sequences and restriction site analysis
for the diagnosis of sickle cell anemia. Science 1985;
230: 1350-1354.
5. Pershing DH. In vitro nucleic acid amplification
techniques. í Diagnostic molecular microbiology.
Principles and applications; bls. 51-87. Ritstj.:
Pershing DH, Smith TF, Tenover FC, White TJ. 1993.
American society for microbiology, Washington D.C.
6. Torfason E, Kristjánsson M. Diagnosis of enteroviral
infection of the central nervous system, using reverse
transcription and senii-nested polymerase chain
reaction (PCR). Abstract, XXXIV. Interscience
conference on antimicrobial agents and chemotherapy
(ICCAC). Orlando, Florida. 1994.
7. Torfason E, Kristjánsson M, Bergsdóttir A. Diagnosis
of enteroviral infection of the central nervous system,
using reverse transcription and semi-nested
polymerase chain reaction (PCR). Abstract, VII.
international conference of comparative and applied
virology. Montreol, Quebec. 1994.
8. Steingrímsson, Ó., Ólafsson, J.H., Karlsson, S.M.,
Pálsdóttir, R. Clinical Evaluation of a Rapid
Polynterase Chain Reaction (PCR) Assay for the
Detection of Chlamydia trachomatis in Specimens
from High Risk Patients. Hefur verið sent til birtingar.
9. Vogels WHM, Van Voorst Vader PC, Schröder FP.
Chlamydia trachomatis Infection in a high risk
population: Comparison of polymerase chain reaction
and cell culture for diagnosis and follow-up. J Clin
Microbiol 1993;31:1103-7.
10. Jaschek G, Gaydos CA, Welsh LE, Quinn TC. Direct
detection of Chlamydia trachomatis in urine
specimens front symptomatic and asymptomatic men
by using a rapid polymerase chain reaction assay. J
Clin Microbiol 1993;31:1209-12.
11. Bauwens JE, Clark AM, Stamm WE. Diagnosis of
Chlamydia trachomatis endocervical infections by a
commercial polymerase chain reaction assay. J Clin
Microbiol 1993;31:3023-27.
12. Bauwens JE, Clark AM, Loeffelholz MJ, Herman
SA, Stamm WE. Diagnosis of Chlamydia trachomatis
urethritis by polymerase chain reaction of first-catch
urine. J Clin Microbiol 1993;31:3013-16.
LÆKNANEMINN 2 1994 47. árg.
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