Fróðskaparrit - 01.01.1953, Blaðsíða 90

96
Rannsóknir um reyðblóðkroppanoringina
In section I. a comparison is made between the production
of erythrocytes in 100 %, 80 %, 50 %, 40 %, 30 10 %,
5 %, 2l/2 % oxygen and in ordinary atmospheric air (20,
95 % 02). The results of some of the experiments are seen
in Fig. 2—7: (Fig. 2 Production of erythrocytes in vitro
in air compared with nitrogen containing 2.5 % 02. —
Fig. 3—7. Production of erythrocytes in vitro in air com-
pared with nitrogen containing 5 % 02). The total results
are shown in tables (Table 1—8), and the final results can
easily be read off from Curves (Fig. 8—9) which indicate
that the maximum production of erythrocytes was seen at
10—30 % of oxygen. The production was inhibited at 40 %
and 5 % of oxygen and completely arrested at 50 % and
2/2 %• Thus the optimal production was observed at the
physiological oxygen tension.
In section II. the production of erythrocytes was similarly
examined in 3 % and 6 % of carbon dioxide in relation to
atmospheric air (Table 9—12).
A special investigation was made to see what influence
pH has on bone marrow cells in nitro. These results
may be seen in Table 13 in addition all the results
are seen in a co-ordinate system; this co-ordinate system
is seen in Figure 10 from which it is evident that increasing
concentrations of carbon dioxide inhibit the formation of
red blood cells from bone marrow in vitro. An inhibition of
the same magnitude is seen when the pH in the solution is
altered to values corresponding to those found in the samples
with increased carbon dioxide. It may be concluded thal
carbon dioxide has now effect on its own, but that the effect
observed is due to the resultant increased hydrogen ion con-
centration in the medium.
It must, however, be emphasized that these results from
experiments in vitro do not exclude the possibility of de-
creased oxygen tension or increased carbon dioxide tension
playing a part in the formation of red blood cells in vivo.