Fróðskaparrit - 01.01.1953, Page 89

Rannsóknir um reyðbióðkroppanóringina
95
kolsýran mundi dyggiliga eggja afturgerð reyðblóðkropp-
anna.
Á 10. mynd eru sett upp úrslitini av royndum við 0 %
C02, 3 °/c og 6 % C02.
Av teim trimum atvoldum til eggjan, sum standa í boði:
1) lágt súrevnistrýst, 2) hogt kolsýrutrýst 3) og nógv súrga,
tykist tað, at vit kunnu gevast við teimum báðum seinastu.
Vit kundu eftir hesum væl trúð, at tað er hitt lága súrevnis-
trýstið, sum í livandi verum óbeinleiðis stýrir blóðgerðini,
t. d. eftir nervalagnum. Spurningurin, ið nú liggur fyri,
er, um tað í lívroyndum fer at bera til at játta ella nokta
fyri hesi tilætlan. — Hetta stendur til komandi rannsóknir
at gera av.
SUMMARY
ín two previous essays (Magnussen 1949, 1950) the
author presented in condensed form the results of studies
on the influence of oxygen tension, carbon dioxide tension
and hydrogen ion concentration on the production of erythro-
cytes in vitro. The present article gives a more exhaustive
account of the difficulties bound up with such investigations
and describes in more detail the author’s technique which
mainly differs from that of Osgood and Plum as follows:
1. The investigations were made in Warburg vessels in
water, both at 38° C.
2. The vessels were kept in constant motion to prevent
sedimentation (Fig. 1).
3. Agglutinations were avoided by using serum from
blood kept at 2°—4° for 24 hours, and then the serum
must not show any signs of hemolysis. Moreover the su-
spension must be kept for a period of 20 minutes before
serum is added.